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p jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p jak2
    P Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+jak2/Jak2+XP+Rabbit+mAb/pm41910173-335-34-36
    Average 96 stars, based on 1259 article reviews
    p jak2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Palmitoylation by ZDHHC4 inhibits TRPV1-mediated nociception
    Article Snippet: Rabbit anti-JAK2 , Cell Signaling Technology , Cat#3230.

    Incubation:

    Article Title: Danggui Shaoyao San attenuates depressive-like behaviors in mice via TLR4/NF-κB p65/JAK-STAT3/AKT-GSK3β signaling pathways: modulation of hippocampal neurogenesis and neuroinflammation
    Article Snippet: Aliquots of protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequently transferred to a polyvinylidene difluoride membrane (IPVH00010, Millipore). .. The membranes were closed with protein-free rapid closure solution (G2052-500 ML, Servicebio) for 5 min at 4 °C with anti-Mouse anti-GAPDH (Cell signaling, 97166, 1: 1000), Mouse anti-β-Actin (Cell signaling, 3700, 1:1000), Rabbit anti-p-stat3 (Cell signaling, 9145, 1:1000), Rabbit anti-stat3 (Cell signaling, 30835, 1:1000), Rabbit anti-NF-κB p65 (Cell signaling, 8242, 1:1000), Rabbit anti-p-NF-κB p65 (Cell signaling, 3033, 1:1000), Rabbit anti-p-AKT (Cell signaling, 9271, 1:1000), Rabbit anti-AKT (Cell signaling, 9272, 1:1000), Rabbit anti-p-JAK2 (Affinity, AF3024, 1:1000), Rabbit anti-JAK2 (Affinity, AF6022, 1:1000), Rabbit anti-TLR4 (Cell signaling, 82658, 1:1000), Rabbit anti-p-GSK3β (Cell signaling, 9336, 1:1000), and Rabbit anti-GSK3β (Cell signaling, 12456, 1:1000) were incubated overnight with primary antibody shaking. .. Afterward, the membranes were incubated with secondary antibody HRP-conjugated Goat Anti-Rabbit IgG(H+L) (Wuhan Sanying, SA00001-2, 1:5000) or HRP-conjugated Goat Anti-Mouse IgG (Wuhan Sanying, SA00001-1-A, 1:5000) for 1 h at room temperature.

    Article Title: Interleukin-6 secreted by tumor-associated macrophages promotes proliferation and migration through JAK2/STAT3 signaling pathway in human prostate cancer cells
    Article Snippet: Samples were homogenized in a lysis buffer, and equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Cytiva). .. Membranes were blocked with 3 % BSA and incubated with primary antibodies: mouse anti-β-actin (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-JAK2 (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phosphorylated JAK2(Cell Signaling Technology), rabbit anti-STAT3 (Cell Signaling Technology), and rabbit anti-phosphorylated STAT3 (Cell Signaling Technology). .. After washing, the membranes were probed with HRP-conjugated goat anti-mouse or anti-rabbit IgG (Bethyl Laboratories Inc., Montgomery, TX, USA).

    Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
    Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).

    Article Title: IL-6 secreted by tumor-associated macrophages promotes proliferation and migration through JAK2/STAT3 signaling pathway in human prostate cancer cells
    Article Snippet: Samples were homogenized in a lysis buffer, and equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Cytiva). .. Membranes were blocked with 3 % BSA and incubated with primary antibodies: mouse anti-β-actin (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-JAK2 (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phosphorylated JAK2(Cell Signaling Technology), rabbit anti-STAT3 (Cell Signaling Technology), and rabbit antiphosphorylated STAT3 (Cell Signaling Technology). .. After washing, the membranes were probed with HRP-conjugated goat anti-mouse or anti-rabbit IgG (Bethyl Laboratories Inc., Montgomery, TX, USA).

    Blocking Assay:

    Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
    Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).

    Labeling:

    Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
    Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).



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    Cell Signaling Technology Inc phospho jak2 tyr1007 1008
    Effects of STG on the <t>JAK2/STAT3</t> signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.
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    Cell Signaling Technology Inc jak2
    Effects of STG on the <t>JAK2/STAT3</t> signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.
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    Image Search Results


    Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing

    The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing, Marker, Staining

    Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques:

    Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing

    The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing, Marker, Staining

    Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: